RNA Isolation from Purified Rice Necrosis Mosaic Virus Particles

Spectroscopic Studies on Purified Particles and Isolated RNA of Cucumber Mosaic Virus

Cucumber mosaic virus (CMV) is an isometric virus which infects a wide range of hosts in the temperate regions of the world (Kaper & Waterworth, 1981). CMV genomic R N A s consist of four components (mol. wt. 1.09, 0-98, 0.71 and 0.33, all x 106). The three largest components carry all the information required for virus replication, whereas the fourth component corresponds to the part of the ge...

Solubility Studies on Purified Tobacco Mosaic Virus

Different samples of purified tobacco mosaic virus show a relatively wide variation in solubility in ammonium sulfate solution. This variation and the type of solubility curve obtained in the presence of varying amounts of solid phase show that the purified virus whether isolated by mild treatment with ammonium sulfate or by ultracentrifugation is not a homogeneous chemical substance but contai...

Isolation of virus like particles from fecal material obtained from diarrheal calves

Isolation of single stranded DNA from purified hepatitis A-virus.

Hepatitis A-virus was purified from human stools by three purification steps. Virus was identified by radioimmunoassay and purity monitored with immune electron microscopy. Virus particles, serologically and morphologically identical, banded in CsCl in two density ranges at 1.31-1.34 g/cm3 and at 1.41-1.43 g/cm3. Virions of density 1.31-1.34 g/cm3 were shown to contain single-stranded DNA of di...

Characterization of Celery Mosaic Virus from Celery in Tehran Province

Background and Aims: Celery mosaic virus (CeMV) is one of the causal agents of viral diseases in celery (Apium graveolens). CeMV is a member of the Potyvirus genus in Potyviridae family. The virus is naturally transmitted by aphids in a non-persistent manner. During growing season 2006-2007 viral disease symptoms were observed in celery fields grown in Tehran Province (Bage daneshkade, Mohamads...